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突出细胞的生命意义与整体性，探索生命奥秘的重任。
内容简介
本书反映了细胞的整体性，更加突出了细胞的生命意义。生命是自然界物质存在的最高形式，探索生命的奥秘无疑是科学家的重任。生命是细胞的运动形式，细胞生物学家应把认识细胞与生命现象紧密联系在一起。
章节目录
版权信息
Author's Comments
Chapter 1 Microscopy
1.1 Introduction:Light Microscope—Resolution vs Magnification
1.2 Bright Field,Dark Field,Phase Contrast
1.3 Fluorescence Microscopy and Confocal Microscopy
1.4 Electron Microscopy
1.5 Exercise:Use of a Bright Field Microscope
1.6 Exercise:Examination of Different Cell Types
Chapter 2 Subcellular Structures
2.1 Introduction:Cells,Organelles and Vital Staining
2.2 Exercise:Vacuole in Animal Cells
2.3 Exercise:Staining of Mitochondria in Human Cheek Epithelial Cells
2.4 Immuno-Staining and Fluorescent Labeling
2.4.1 Immuno-Staining
2.4.2 Fluorophores and Fluorescent Labeling
2.5 Exercise:Immunofluorescent Analysis of Microtubules
Chapter 3 Centrifugation and Subcellular Fractionation
3.1 Introduction:Equilibrium Density Gradient Centrifugation and Differential Sedimentation
3.2 Exercise:Purification of Nuclear and Mitochondria by Differential Sedimentation
3.3 Exercise:Assay of Fractions for Succinate Dehydrogenase(SDH)
Chapter 4 Cell Membrane and Cross-Membrane Transport
4.1 Introduction:Membranes and Its Properties
4.2 Exercise:Osmosis
4.3 Exercise:Hemolysis
4.4 Exercise:Pinocytosis
Chapter 5 Cell Cycle and Cell Division
5.1 Introduction:Types of Cell Division
5.2 Exercise:Study of Different Stages of Mitosis in Onion Root Tip Cells
5.3 Exercise:Study of Different Stages of Meiosis in Mouse Testis Cells
Chapter 6 Cytogenetics and Karyotyping
6.1 Introduction:Karyotyping
6.2 Exercise:Short Term Culture of White Blood Cells and Preparation of Metaphase Chromosomes
6.3 G-and C-Banding of Metaphase Chromosomes
6.3.1 Exercise:G-Banding of Metaphase Chromosomes
6.3.2 Exercise：C-Banding of Metaphase Chromosomes
6.4 Exercise:Karyotyping
Chapter 7 Cell Culture
7.1 Introduction:Principles of Animal Cell Culture
7.2 Sterilization Techniques
7.3 Exercise:Establishment of a Primary Culture
7.4 Exercise:Cell Viability Assay by Trypan Blue Exclusion
7.5 Exercise:MTT Assay for Cell Viability
7.6 Exercise:Mammalian Cell Transfection
7.6.1 Exercise：Lipofectamine Mediated Mammalian Cell Transfection
7.6.2 Exercise:Transfecting Mammalian Cells by Electroporation
7.7 Exercise:PEG Induced Fusion of Erythrocytes
Chapter 8 Apoptosis Analysis
8.1 Introduction:The Characteristic Cellular Changes of Apoptosis
8.2 Exercise:Induction of Apoptosis
8.3 Exercise:Vital Staining and Detection of Chromatin Condensation
8.4 Exercise:Detection of DNA Fragmentation
Chapter 9 Flow Cytometry
9.1 Introduction:Flow Cytometry and Signal Processing
9.2 Exercise:Cell Cycle Analysis by Propidium Iodide(PⅠ)Staining
9.3 Exercise:Cell Sorting by Fluorescence-Activated Cell Sorting(FACS)
Chapter 10 Cellular Immunology:Enzyme-Linked Immunosorbent Assay(ELISA)
10.1 Introduction:The History and the Application
10.2 Exercise:Direct ELISA(Also Known as Sandwich ELISA)
10.3 Exercise:Capture ELISA
Chapter 11 Molecular Cell Biology
11.1 Exercise:DNA Extraction and Purification
11.2 Exercise:Determination of DNA Concentration Using the Spectrophotometer
11.3 Exercise:Total RNA Isolation from Cells in Culture
11.4 Standard PCR
11.4.1 Introduction:Principles of PCR
11.4.2 Exercise:PCR Amplification of DNA
11.4.3 Exercise:Two-Step RT-PCR Amplification of β-Actin
11.5 Exercise:Southern Blot Using 32P Labeled dGTP
11.6 Measurement of Cell Protein Content
11.6.1 Introduction:Bradford Assay
11.6.2 Exercise:Protein Assay and Measurement of Protein Content of Cells
11.7 Protein Extraction and Purification
11.7.1 Introduction:The Principles for Protein Extraction
11.7.2 Exercise:Whole Cell Extract Preparation
11.8 Exercise:Protein Electrophoresis
11.8.1 Introduction:Protein Electrophoresis and SDS-PAGE
11.8.2 Exercise:Protein Separation on SDS-PAGE
11.9 Protein Analysis and Detection
11.9.1 Exercise:Visualization of Proteins in Gels
11.9.2 Exercise:Semi-Dry Transfer of Protein onto Membrane
11.9.3 Exercise:Western Blot
Appendix: Chemical Preparations
分子细胞生物学实验教程是2013年由暨南大学出版社出版,作者邹奕 主编。
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